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KMID : 1007520090180010223
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Food Science and Biotechnology
2009 Volume.18 No. 1 p.223 ~ p.227
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Triterpenoids from the Fruits of Cornus kousa Burg. as Human Acyl-CoA: Cholesterol Acyltransferase Inhibitors
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Lee Dae-Young
Jung Lakoon
Lyu Ha-Na
Jeong Tae-Sook
Lee Youn-Hyung
Baek Nam-In
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Abstract
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The fruits of Cornus kousa Burg. were extracted with 80% aqueous methanol (MeOH) and the concentratedextract was partitioned with ethyl acetate (EtOAc), n-butanol (n-BuOH), and H2O. From the EtOAc fraction, 5 triterpenoidswere isolated through repeated silica gel (SiO2), octadecyl silica gel (ODS), and Sephadex LH-20 column chromatography(c.c.). These compounds were determined to be ursolic acid (1), corosolic acid (2), taraxasterol (3), betulinic acid (4), andbetulinic aldehyde (5) on the basis of their spectroscopic data including electronic ionization mass spectrometry, ultravioletspectroscopy, infrared spectroscopy, and nuclear magnetic resonance. This is the first reported isolation of these compoundsfrom this plant. Also, compounds 1, 3, 4, and 5 show a relatively high inhibitory activity against human acyl-CoA: cholesterolacyltransferase-1 (hACAT-1) with inhibition values of 52.8±0.7, 91.1±0.4, 93.0±0.7, and 96.2±0.2% at a concentration of100μM, respectively.
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KEYWORD
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Cornus kousa, ursolic acid, corosolic acid, taraxasterol, betulinic acid, betulinic aldehyde, acyl-CoA:cholesterolacyltransferase
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